Partial change of the ts-phenotype of cold-adapted influenza virus strain grown in canine kidney cells in the presence of trypsin.
Identifieur interne : 002633 ( Main/Exploration ); précédent : 002632; suivant : 002634Partial change of the ts-phenotype of cold-adapted influenza virus strain grown in canine kidney cells in the presence of trypsin.
Auteurs : L M Garmashova ; G F Leontieva ; Dubrovina TYa ; G I AleksandrovaSource :
- Acta virologica [ 0001-723X ] ; 1982.
Descripteurs français
- KwdFr :
- Adaptation physiologique, Animaux, Basse température, Bovins, Cellules cultivées, Chiens, Embryon de poulet, Lignée cellulaire, Protéines virales (biosynthèse), Rein, Réplication virale (), Trypsine (pharmacologie), Virus de la grippe A (), Virus de la grippe A (croissance et développement), Virus de la grippe A (métabolisme).
- MESH :
- biosynthèse : Protéines virales.
- croissance et développement : Virus de la grippe A.
- métabolisme : Virus de la grippe A.
- pharmacologie : Trypsine.
- Adaptation physiologique, Animaux, Basse température, Bovins, Cellules cultivées, Chiens, Embryon de poulet, Lignée cellulaire, Rein, Réplication virale, Virus de la grippe A.
English descriptors
- KwdEn :
- Adaptation, Physiological, Animals, Cattle, Cell Line, Cells, Cultured, Chick Embryo, Cold Temperature, Dogs, Influenza A virus (drug effects), Influenza A virus (growth & development), Influenza A virus (metabolism), Kidney, Trypsin (pharmacology), Viral Proteins (biosynthesis), Virus Replication (drug effects).
- MESH :
- chemical , biosynthesis : Viral Proteins.
- chemical , pharmacology : Trypsin.
- drug effects : Influenza A virus, Virus Replication.
- growth & development : Influenza A virus.
- metabolism : Influenza A virus.
- Adaptation, Physiological, Animals, Cattle, Cell Line, Cells, Cultured, Chick Embryo, Cold Temperature, Dogs, Kidney.
Abstract
The cold-adapted temperature-sensitive (ts) influenza virus strain A/Leningrad/134/17/57 (H2N2) multiplied well at 32 degrees C (optimal temperature); lower titres of infectious virus were obtained in developing chick embryos at 40 degrees C. In a canine kidney (MDCK) cell line and in primary calf kidney (CK) cells an increased reproduction of the virus was found at 40 degrees C especially in the presence of trypsin. The ratios of virus titres obtained at optimal versus higher temperatures (RCT40) were by 1,000 times lower than those found in chick embryos. Polyacrylamide gel electrophoresis revealed a comparable synthesis of the cold-adapted influenza virus strain polypeptides HA, NP, M and NS in MDCK cells, regardless whether they were incubated at optimal or non-permissive temperatures.
PubMed: 6127930
Affiliations:
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Le document en format XML
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the presence of trypsin.</title><author><name sortKey="Garmashova, L M" sort="Garmashova, L M" uniqKey="Garmashova L" first="L M" last="Garmashova">L M Garmashova</name></author><author><name sortKey="Leontieva, G F" sort="Leontieva, G F" uniqKey="Leontieva G" first="G F" last="Leontieva">G F Leontieva</name></author><author><name sortKey="Dubrovina Tya" sort="Dubrovina Tya" uniqKey="Dubrovina Tya" last="Dubrovina Tya">Dubrovina TYa</name></author><author><name sortKey="Aleksandrova, G I" sort="Aleksandrova, G I" uniqKey="Aleksandrova G" first="G I" last="Aleksandrova">G I Aleksandrova</name></author></analytic><series><title level="j">Acta virologica</title><idno type="ISSN">0001-723X</idno><imprint><date when="1982" type="published">1982</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Physiological</term><term>Animals</term><term>Cattle</term><term>Cell Line</term><term>Cells, Cultured</term><term>Chick Embryo</term><term>Cold Temperature</term><term>Dogs</term><term>Influenza A virus (drug effects)</term><term>Influenza A virus (growth & development)</term><term>Influenza A virus (metabolism)</term><term>Kidney</term><term>Trypsin (pharmacology)</term><term>Viral Proteins (biosynthesis)</term><term>Virus Replication (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adaptation physiologique</term><term>Animaux</term><term>Basse température</term><term>Bovins</term><term>Cellules cultivées</term><term>Chiens</term><term>Embryon de poulet</term><term>Lignée cellulaire</term><term>Protéines virales (biosynthèse)</term><term>Rein</term><term>Réplication virale ()</term><term>Trypsine (pharmacologie)</term><term>Virus de la grippe A ()</term><term>Virus de la grippe A (croissance et développement)</term><term>Virus de la grippe A (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Trypsin</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Influenza A virus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Trypsine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adaptation, Physiological</term><term>Animals</term><term>Cattle</term><term>Cell Line</term><term>Cells, Cultured</term><term>Chick Embryo</term><term>Cold Temperature</term><term>Dogs</term><term>Kidney</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adaptation physiologique</term><term>Animaux</term><term>Basse température</term><term>Bovins</term><term>Cellules cultivées</term><term>Chiens</term><term>Embryon de poulet</term><term>Lignée cellulaire</term><term>Rein</term><term>Réplication virale</term><term>Virus de la grippe A</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The cold-adapted temperature-sensitive (ts) influenza virus strain A/Leningrad/134/17/57 (H2N2) multiplied well at 32 degrees C (optimal temperature); lower titres of infectious virus were obtained in developing chick embryos at 40 degrees C. In a canine kidney (MDCK) cell line and in primary calf kidney (CK) cells an increased reproduction of the virus was found at 40 degrees C especially in the presence of trypsin. The ratios of virus titres obtained at optimal versus higher temperatures (RCT40) were by 1,000 times lower than those found in chick embryos. Polyacrylamide gel electrophoresis revealed a comparable synthesis of the cold-adapted influenza virus strain polypeptides HA, NP, M and NS in MDCK cells, regardless whether they were incubated at optimal or non-permissive temperatures.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Aleksandrova, G I" sort="Aleksandrova, G I" uniqKey="Aleksandrova G" first="G I" last="Aleksandrova">G I Aleksandrova</name><name sortKey="Dubrovina Tya" sort="Dubrovina Tya" uniqKey="Dubrovina Tya" last="Dubrovina Tya">Dubrovina TYa</name><name sortKey="Garmashova, L M" sort="Garmashova, L M" uniqKey="Garmashova L" first="L M" last="Garmashova">L M Garmashova</name><name sortKey="Leontieva, G F" sort="Leontieva, G F" uniqKey="Leontieva G" first="G F" last="Leontieva">G F Leontieva</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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